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M94A2725.TXT
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1994-10-25
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Document 2725
DOCN M94A2725
TI Performance of the Red-Dot HIV 1 & 2 assay.
DT 9412
AU Abdel-Hamid M; Constantine NT; Dept. Pathology, University of Maryland
School of Medicine,; Baltimore.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):239 (abstract no. PB0387). Unique
Identifier : AIDSLINE ICA10/94369850
AB OBJECTIVES: To determine the suitability and performance of a new rapid
assay to detect antibodies to HIV 1 & 2 in a hospital setting in
Baltimore, MD. METHODS: A total of 91 sera (50 EIA negative, 39 EIA
repeatedly reactive/Western blot (WB) confirmed, and 2 WB indeterminate)
were tested by the Red-Dot HIV 1 & 2 rapid assay (CDI, CA, USA). In
addition, 9 samples were included that were EIA false positive (WB
negative). The Red-Dot test is a rapid membrane based assay which
incorporates a synthetic peptide antigen and a protein A colloidal gold
conjugate. Samples which exhibited discrepant results between the two
screening tests were retested by all assays to ensure that technical
error had not occurred. RESULTS: 39 sera produced reactive results by
both tests and were confirmed by Western blot (WB). The Red-Dot test
produced 3 false positive results (detected 56 of 59 true negatives),
and no false negative results (0/39), thus yielding a specificity of 95%
and a sensitivity of 100%. Both of the WB indeterminate samples produced
reactive results by EIA, while only one produced a reactive result by
the Red-Dot. DISCUSSION AND CONCLUSIONS: The Red-Dot HIV 1 & 2 rapid
assay was easy to perform, required just minutes to complete, exhibited
an excellent sensitivity and an acceptable specificity. This study
should be expanded to include a larger sample size in order to verify
the assay's performance.
DE *AIDS Serodiagnosis *Blotting, Western Human HIV
Seropositivity/*DIAGNOSIS/IMMUNOLOGY HIV-1/*IMMUNOLOGY
HIV-2/*IMMUNOLOGY *Immunoenzyme Techniques Predictive Value of Tests
MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).